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Scientists develop sensitive BPA detection method

By Rod Addy , 16-Jan-2013
Last updated on 16-Jan-2013 at 13:31 GMT2013-01-16T13:31:19Z

Scientists develop sensitive BPA detection method

Scientists have developed a highly sensitive method of detecting the food contact material bisphenol-A (BPA) in food and water.

The technique is based on synthesis of a poly-L-lysine (PLL)-hapten conjugate as a coating antigen and was published online for the journal Analytical Methods.

The performance of the PLL–4,4-bis(4-hydroxyphenol) valeric acid (BVA) conjugate in the immunoassay was studied. The established method showed that the PLL–BVA coated format could improve the sensitivity of indirect competitive enzyme-linked immunosorbent assay (icELISA) tests.

The limit of detection was 0.5 nanograms per millilitre and the reliability of the ELISA test was verified using high-performance liquid chromatography.

“These data demonstrated that the established ic-ELISA is a potential technique for detection of BPA,” the researchers concluded.

BPA is used to coat packaging such as aluminium cans and some studies describe it as a risk factor in the development of ailments ranging from cancer to obesity.

Source: Analytical Methods 2013, first published online January 14, 'An indirect competitive enzyme-linked immunosorbent assay for bisphenol-A based on the synthesis of a poly-L-lysine-hapten conjugate as a coating antigen'. Authors: Jianging Zhou, Suging Zhao, Jun Zhang, Yanfei Cai and Lihua Zhou.

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