The technique is based on synthesis of a poly-L-lysine (PLL)-hapten conjugate as a coating antigen and was published online for the journal Analytical Methods.
The performance of the PLL–4,4-bis(4-hydroxyphenol) valeric acid (BVA) conjugate in the immunoassay was studied. The established method showed that the PLL–BVA coated format could improve the sensitivity of indirect competitive enzyme-linked immunosorbent assay (icELISA) tests.
The limit of detection was 0.5 nanograms per millilitre and the reliability of the ELISA test was verified using high-performance liquid chromatography.
“These data demonstrated that the established ic-ELISA is a potential technique for detection of BPA,” the researchers concluded.
BPA is used to coat packaging such as aluminium cans and some studies describe it as a risk factor in the development of ailments ranging from cancer to obesity.
Source: Analytical Methods 2013, first published online January 14, 'An indirect competitive enzyme-linked immunosorbent assay for bisphenol-A based on the synthesis of a poly-L-lysine-hapten conjugate as a coating antigen'. Authors: Jianging Zhou, Suging Zhao, Jun Zhang, Yanfei Cai and Lihua Zhou.