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Novel HPLC Approaches for Carbohydrate Analysis in Beverages and Food

Format: Webinar | Document type: Supplier Webinar

Event Overview:

Carbohydrates are important food components affecting taste and nutrition. The determination of the types and levels of carbohydrates in foods is important for energy evaluation, nutritional labeling, quality control and for identifying possible adulteration.

Separation and detection of carbohydrates can be challenging. Simple carbohydrates are highly polar, uncharged, and as they lack a chromophore, cannot be measured by UV absorbance detectors. This webinar will introduce two novel approaches for carbohydrate analysis based on their separation by either High Performance Liquid Chromatography (HPLC) or High Performance Anion Exchange Chromatography (HPAE). Different detection techniques will also be presented including Pulsed Amperometric Detection (PAD) and Charged Aerosol Detection (CAD). The advantages of these approaches over other methods such as fluorescent derivatization will also be discussed. Examples will include the routine measurement of simple carbohydrates in foods and beverages, the determination of the possible carcinogen hydroxyl-methylfurfural in honey, and rapid method for measurement of carbohydrates in instant and green coffee that shows improvement over AOAC Official Method 995.13.

This webinar will describe the latest chromatographic approaches for the routine measurement of carbohydrates in a variety of foods and beverages. Learn about the universal charged aerosol detection and selective pulsed amperometric detection for the direct determination of carbohydrates in complex matrices. Learn about the advantages of these approaches over other analytical methods and the improved throughput and selectivity these approaches can bring to your lab.

Key Learning Objectives:

  • Different separation and detection technologies for carbohydrate analysis in food and beverage
  • Two novel HPLC approaches for simple carbohydrate analysis, HPAE-PAD and HILIC-CAD.
  • Advantages of HPAE-PAD and HILIC-CAD over other methods for simple carbohydrate analysis

Who Should Attend:

  • Scientists involved in analyzing carbohydrates in the food and beverage industry

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