3M assay given AOAC OMA validation

By Joseph James Whitworth

- Last updated on GMT

3M MDS Salmonella Kit with Media
3M MDS Salmonella Kit with Media

Related tags Molecular detection assay Dna

3M Food Safety’s Molecular Detection Assay Salmonella has been validated through AOAC International as a First Action Official Method of Analysis (OMA) for the detection of the pathogen in selected foods.

The assays use loop-mediated isothermal amplification to amplify nucleic acid sequences with high specificity and sensitivity, combined with bioluminescence to detect the amplification.

They use multiple primers to recognize distinct regions of the genome and a DNA polymerase to provide continuous and rapid amplification.

Foods targeted

John M. David, a global technical services engineer with 3M Food Safety, said the matrices in the validation are pasteurized liquid whole egg, raw ground beef, cooked breaded chicken, raw shrimp, bagged spinach and wet pet food.

He told FoodQualityNews.com that they plan to expand the validation scope with additional matrices, in the case of the 3M Molecular Detection Assay - Salmonella and other Molecular Detection assays.

“The technical rigor of AOAC OMA is well known around the world by the microbiology analytical community and it specifically serves as a sought-after validation by a number of customers.

“By adding AOAC OMA to the Molecular Detection Assay-Salmonella's current AOAC PTM and NF Validation, 3M can offer a range of internationally recognized certifications that our customers require in making decisions.”

OMA validation is AOAC International’s internationally recognized program for chemical, microbial and molecular biological testing methods. Consisting of a joint validation in multiple labs and reviewed by an expert panel, it ascribes a given method as an official method of analysis.

OMA expansion of PTM

The OMA builds on the Performance Tested Method (PTM), which compares new and alternative testing methods with standard reference methods for each food and environmental matrix. PTM tests also include inclusivity and exclusivity strains, as well as a series of tests challenging the robustness of the method’s instrumentation.

In the assay, pyrophosphate ions, generated by amplification of the targeted DNA, and a substrate are enzymatically converted into Adenosine triphosphate (ATP) by ATP-Sulfurylase.

ATP reacts with luciferase to produce light, which is then detected by the 3M Molecular Detection Instrument, indicating the presence of target organism DNA.

“Both amplification and detection occur simultaneously and continuously during the exponential phase providing real time results and a short run time,” ​said David.

“The system is able to provide real-time results in as early as 15 minutes, following an overnight enrichment and simple assay preparation protocol. Negative results are reported at the end of the 75-min instrument run.”

The 3M Molecular Detection Assay Salmonella has received AOAC PTM and AOAC OMA validation as well as NF Validation by AFNOR Certification.

3M Molecular Detection Assay E.coli 0157 has received AOAC PTM validation and NF Validation by AFNOR Certification while their Molecular Detection Assay Listeria has received AOAC PTM validation.

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